| Comments |
P.2421 left column top paragraph:"As for glnS mRNA, although its abundance decreased upon S1 depletion, its half-life was affected by neither the absence of PNPase nor S1 depletion (Fig. 6 Table 1). Thus the decreased mRNA abundance observed upon S1 depletion cannot be imputed to a faster mRNA turnover, as it is, at best, unchanged or, paradoxically, decreases for the pnp mRNA in the presence of PNPase." P.2421 right column bottom paragraph:"PNPase abundance in the pnp+ strain was unaffected by S1 overexpression, as assessed by Western blotting (Fig. 4C). Conversely, S1 overexpression greatly affected pnp mRNA abundance and stability. In the pnp+ strain, S1 overexpression led to an about twofold and 10-fold increase of the monocistronic (2.25 kb long) and polycistronic (5.4 kb long) pnp mRNA levels, respectively (Fig. 7, left panel, lanes 3,8) while the half-life of these transcripts was considerably extended (>10-fold for the monocistronic and about fivefold for the polycistronic mRNA Table 2). However, in the PNPase-deficient strain, S1 overexpression did not further increase the abundance of the 0.3-, 0.7-, and 1.3-kb-long Δpnp-751 mRNAs (Fig. 7, right panel, lanes 3,9) and their half-lives increased only about twofold. To the contrary, the polycistronic 3.4 kb RNA exhibited a 10-fold half-life increase (Table 2)." See notes beneath tables |