Transcription Fidelity In Vitro

Range Table - link
Organism Budding yeast Saccharomyces cerevisiae
Reference Kireeva ML. et al., Transient reversal of RNA polymerase II active site closing controls fidelity of transcription elongation. Mol Cell. 2008 Jun 6 30(5):557-66. doi: 10.1016/j.molcel.2008.04.017. p.561 table 1PubMed ID18538654
Method P.561 caption beneath table 1: "TEC9 [elongation complex containing a 9 nt transcript] was obtained on the template 45C and chased with 5–500 mM CTP or 0.1–5 mM UTP. The error values for kpol and KD estimates were obtained from the hyperbolic fit as described in the Supplemental Experimental Procedures."
Comments
Entered by Uri M
ID 110019