Value |
19.1
Unitless
|
Organism |
Human Homo sapiens |
Reference |
Watanabe et al., Heat-evoked activation of TRPV4 channels in a HEK293 cell expression system and in native mouse aorta endothelial cells. J Biol Chem. 2002 Dec 6 277(49):47044-51. p.47046 figure 2E, caption to figure 2E & right column top paragraphPubMed ID12354759
|
Method |
P.47046 left column bottom paragraph:"To estimate the temperature threshold for TRPV4 activation, [investigators] have simultaneously recorded the changes in bath temperature and current (Fig. 2). At temperatures above 25 °C the TRPV4 current increased exponentially (Fig. 2, A and B)." |
Comments |
P.47046 left column bottom paragraph:"The current plotted as a function of temperature showed hysteresis (Fig. 2 C). Ruthenium red, a selective voltage-dependent blocker of TRPV channels, rapidly inhibited the current (Fig. 2 D). Pooled data from 5 cells are shown in Fig. 2 E. The Q10 value was assessed from the slope of the log(I) versus temperature plot (see Equation 1). Obviously, at low temperatures this slope is small (Q10 = 1.6 ± 1.0) but increases strongly at temperatures above 24 °C to a Q10 value 19.1 ± 1.1. The threshold value of 24 °C was obtained from the crossover of both regression lines. Up to 43 °C no saturation was observed in these experiments. The currents activated by heat are smaller than those during stimulation with 4αPDD, indicating that further heating may recruit even more channels." |
Entered by |
Daniel Ramot |
ID |
100325 |