| Value |
1.71E+15
bases/µg DNA
|
| Organism |
Generic |
| Reference |
J F Kukowska-Latallo, A U Bielinska, J Johnson, R Spindler, D A Tomalia, J R Baker, Jr, Efficient transfer of genetic material into mammalian cells using Starburst polyamidoamine dendrimers. Proc Natl Acad Sci U S A. 1996 May 14 93(10): 4897–4902. p.4898 left column top paragraphPubMed ID8643500
|
| Method |
Abstract: "Starburst polyamidoamine dendrimers are a new class of synthetic polymers with unique structural and physical characteristics. These polymers were investigated for the ability to bind DNA and enhance DNA transfer and expression in a variety of mammalian cell lines. Twenty different types of polyamidoamine dendrimers were synthesized, and the polymer structure was confirmed using well-defined analytical techniques. The efficiency of plasmid DNA transfection using dendrimers was examined using two reporter gene systems: firefly luciferase and bacterial beta-galactosidase. The transfections were performed using various dendrimers, and levels of expression of the reporter protein were determined. Highly efficient transfection of a broad range of eukaryotic cells and cell lines was achieved with minimal cytotoxicity using the DNA/dendrimer complexes." |
| Comments |
P.4898 left column top paragraph: "Ratios of nucleic acid to dendrimer were based on the electrostatic charge present on each component: the number of phosphate groups in the nucleic acid compared with the number of terminal NH2 groups on a dendrimer. For example, given that the number of bases in 1.0µg of DNA is 1.71×10^15, ≈1.71×10^15 negative charges are present per 1.0µg of DNA, whereas a G7(NH3) dendrimer has ≈2.65×10^15 Charges per µg." |
| Entered by |
Nina |
| ID |
113034 |