| Range |
small cells (∼8 μm long) ~80: long cells (>14 μm long) ~140 patches/cell
|
| Organism |
Fission yeast Schizosaccharomyces pombe |
| Reference |
Berro J, Pollard TD. Local and global analysis of endocytic patch dynamics in fission yeast using a new "temporal superresolution" realignment method. Mol Biol Cell. 2014 Nov 5 25(22):3501-14. doi: 10.1091/mbc.E13-01-0004. p.3508 left column 3rd paragraphPubMed ID25143395
|
| Method |
Abstract:"[Investigators] introduce new tools to track and count endocytic patches in fission yeast to increase the quality of the data extracted from quantitative microscopy movies." |
| Comments |
p.3508 left column 3rd paragraph:"The number of patches is proportional to the cell length: [Investigators’] estimate of the number of patches in unsynchronized wild-type cells (Figure 7C) ranges from around 80 patches for small cells (~8 µm long) to ~140 patches for the long cells (>14 µm long). Strikingly, the number of patches per cell is roughly proportional to cell length. This is quite surprising, because the distribution of patches changes during the cell cycle, from initially polarized at the old pole, to polarized to both poles, to localized in the middle around the contractile ring during cytokinesis (Marks and Hyams, 1985 Figure 7A). [Investigators’] finding means that the cell maintains a constant density of patches per unit length even as patch distribution varies across the cell cycle." |
| Entered by |
Uri M |
| ID |
111867 |