Fraction of bacterial cytoplasm that consists of ‘‘bound’’ water (presumably as a hydration layer to macromolecules)
| Value | 20 % |
|---|---|
| Organism | bacteria |
| Reference | Parry BR et al. (2014) The bacterial cytoplasm has glass-like properties and is fluidized by metabolic activity. Cell 156: 183–94. doi: 10.1016/j.cell.2013.11.028. p.187 right column 2nd paragraphPubMed ID24361104 |
| Primary Source | Cayley, S., Lewis, B.A., Guttman, H.J., and Record, M.T., Jr. (1991). Characterization of the cytoplasm of Escherichia coli K-12 as a function of external osmolarity. Implications for protein-DNA interactions in vivo. J. Mol. Biol. 222, 281–300PubMed ID1960728 |
| Comments | "While measuring crowding inside bacterial cells is technically difficult and is subject to uncertainties, estimates suggest that macromolecules occupy 20%– 40% of the bacterial cytoplasm (primary source & Zimmerman and Trach, 1991). An additional 20% of the cytoplasm consists of "bound" water, presumably as a hydration layer to macromolecules (primary source), suggesting that the total excluded volume may range between 40%–60%." |
| Entered by | Uri M |
| ID | 110501 |