| Range |
500 to 700 nm^2/sec
|
| Organism |
Fission yeast Schizosaccharomyces pombe |
| Reference |
Reyes-Lamothe R, Possoz C, Danilova O, Sherratt DJ. Independent positioning and action of Escherichia coli replisomes in live cells. Cell. 2008 Apr 4 133(1):90-102. doi: 10.1016/j.cell.2008.01.044. p.100 left column bottom paragraphPubMed ID18394992
|
| Method |
"Fluorescent C-terminal fusion-protein derivatives of E. coli replisome components, expressed from their endogenous chromosomal promoters, were constructed and their phenotypes assessed by growth, flow cytometry, and microscopy." |
| Comments |
"...the observed rates of replisome movement (mean
step sizes of 100 nm/3 s and a Dapp of (5–7?10^2) nm^2/s)
are of the order one would predict for the replication of
~450?10 kb independent chromosomal domains since such
domains would have a diameter of 100 nm and take 12 s to
replicate." |
| Entered by |
Ron Milo - Admin |
| ID |
109253 |