Value |
19
nts/sec
Range: Table - link nts/sec
|
Organism |
Bacteria Escherichia coli |
Reference |
Proshkin S, Rahmouni AR, Mironov A, Nudler E. Cooperation between translating ribosomes and RNA polymerase in transcription elongation. Science. 2010 Apr 23 328(5977):504-8. p.505 table 1 and middle column top paragraphPubMed ID20413502
|
Method |
"To calculate the elongation rate, the
Escherichia coli culture was induced with IPTG,
and the time that elapsed between the appearance
of a specific hybridization signal from
probes complementary to the 5' and 3' segments
of the lacZ transcript was determined by dotblot
hybridization (Fig. 1 and fig. S1) (5, 6)." |
Comments |
"The speed of transcription elongation in CH184 was remarkably slow (only 19 nt/s), but accelerated to 30 nt/s when Sm was added (Fig. 1C), which perfectly matched the translation elongation rates with and without Sm (Table 1)." |
Entered by |
Uri M |
ID |
108491 |