| Comments |
"As a measure of catalytic proficiency and a benchmark for the design of potential transition-state analogue inhibitors, the value of Ktx ([transition-state binding affinity,] obtained by dividing the rate constant of the uncatalyzed reaction (knon) by kcat/Km: see Figure 2) is of special interest. In Figure 6, the length of each vertical bar represents the value of Ktx. These values range from 10^-8 to 10^-23 M, setting an upper limit (Figure 2) on the dissociation constant of the enzyme's complex with the altered substrate in the transition state." knon=non enzymatic rate constant |