The effect of mutations in conserved decoding center nucleotides on canonical translation and trans-translation
| Range | Table - link Sec^-1 |
|---|---|
| Organism | Bacteria Escherichia coli |
| Reference | Miller MR, Liu Z, Cazier DJ, Gebhard GM, Herron SR, Zaher HS, Green R, Buskirk AR. The role of SmpB and the ribosomal decoding center in licensing tmRNA entry into stalled ribosomes. RNA. 2011 Jul 27 7th page table 3PubMed ID21795410 |
| Method | Peptide-bond formation rate reactions were carried out at 37°C by mixing equal volumes of initiation complexes with either the ternary or quaternary complexes described above. The reactions were stopped at desired time points by addition of KOH to a final concentration of 0.3 M. |
| Entered by | Uri M |
| ID | 106571 |