Comments |
"Two reports provide an independent, yet coherent, estimation of the total number of ribosomes: 187,000±56,000 [BNID 100267] and 200,000 [ref 57] molecules per cell. In this study, [researchers] decided to set W to 200,000. The value of 85% was established for the parameter p, as stated in experimental studies [refs 61,62]. The number of all transcripts
in a cell is more problematic. Many contemporary studies assume
that a yeast cell contains 15,000 mRNAs per cell on average [refs 27,63],
which is based on estimations done over 30 years ago [ref 64]. Current
research, based on more up-to-date techniques (e.g., in situ hybridisation or GATC-PCR) argues that the number should be
at least doubled [ref 65] or even quadrupled [ref 62]. [Researchers] decided to use
the value of X situated between these estimates and equal to 36,000.
This number was also confirmed by other studies [Miura et al., 2008 PMID 19040753 p.9 right column 2nd paragraph & p.10 left column 2nd paragraph, method-Generalized Adaptor-Tagged Competitive PCR (GATC-PCR)]." See BNID 102988, 103023 |