| Value |
7.34
Unitless
|
| Organism |
Human Homo sapiens |
| Reference |
Llopis J, McCaffery JM, Miyawaki A, Farquhar MG, Tsien RY. Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins. Proc Natl Acad Sci U S A. 1998 Jun 9 95(12):6803-8. p.6805 fig.3bPubMed ID9618493
|
| Method |
Compartment-
Specific Expression of EYFP in Mammalian Cells. Fluorescence microscopy in living intact cell. Value extracted visually from fig. 3b. |
| Comments |
"Fluorescence of HeLa cells transfected with the gene encoding EYFP (Fig. 2) was diffusely distributed in the cytosol and nucleus (Fig. 3a), as expected for a protein of 27kDa, which can pass through nuclear pores. EYFP expressed in cells was sensitive to pH changes because perfusion with the permeant base NH4Cl (19) caused an increase in fluorescence (rise in pH), which reversed on wash-out, overshoot to a lower pH, and gradually rose to control levels (Fig. 3b)." Similarly p.6807 fig.5d shows ph=7.4. See BNID 106187 |
| Entered by |
Uri M |
| ID |
105943 |