Calculated parameters of sNRP movements derived from FRAP measurements in BAC HeLa and E3 U2-OS cell lines
| Range | Table - link |
|---|---|
| Organism | Human Homo sapiens |
| Reference | Huranová M, Ivani I, Benda A, Poser I, Brody Y, Hof M, Shav-Tal Y, Neugebauer KM, Stanek D. The differential interaction of snRNPs with pre-mRNA reveals splicing kinetics in living cells. J Cell Biol. 2010 Oct 4 191(1):75-86. p.80 table 2PubMed ID20921136 |
| Method | FRAP and FCS-a technique that focuses a laser beam on a spot of interest in the nucleoplasm and monitors fluctuations in fluorescence intensity over time. The intensity record is transformed into an autocorrelation function that assesses the diffusion correlation time of the detected molecules. |
| Comments | HeLa cell lines that express from BACs the GFP-tagged snRNP proteins. See note under table. |
| Entered by | Uri M |
| ID | 105700 |