| Range |
67-98 %
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Schicklmaier P, Moser E, Wieland T, Rabsch W, Schmieger H. A comparative study on the frequency of prophages among natural isolates of Salmonella and Escherichia coli with emphasis on generalized transducers. Antonie Van Leeuwenhoek. 1998 Jan73(1):49-54. p. 53 table 4PubMed ID9602278
|
| Method |
All strains studied for phage release were cultivated
starting with single colonies to be sure that the phages
were not contaminants. Nevertheless, there might be
presumed that researchers were dealing with phages carried
along on the outside of the cell or in a pseudolysogenic
state. (Both possibilities would not diminish the
importance of the abundance of transducing phage as
vehicles for gene transfer in bacterial populations in
nature). In order to demonstrate that the released (P22
related) phages can really be traced back to integrated
prophages, they hybridized EcoRI digested phage DNA
and equally treated chromosomal DNA of their host
strains against total DNA of phage P22 as a probe. |
| Comments |
Researchers studied
two collections of the species Escherichia coli: the
ECOR collection (Ochman & Selander 1984), a reference
collection analogous to SARA and SARB, and an
additional collection (Gießen E. coli collection) comprising
isolates from cattle, collected in 1993 and 1994
at the Veterinary Institute of the University of Gießen,
Germany 67.2% for ECOR and 97.5% for Gießen, 84/107 strains in total. These values after induction with mitomycin C. Before induction levels were 49% and 77.5%, respectively. |
| Entered by |
Uri M |
| ID |
105605 |