| Value |
19.2
nm/sec
Range: ±6.1 nm/sec
|
| Organism |
Mammals |
| Reference |
McConnell RE, Tyska MJ. Myosin-1a powers the sliding of apical membrane along microvillar actin bundles. J Cell Biol. 2007 May 21 177(4):671-81 p. 673 left column bottom paragraphPubMed ID17502425
|
| Method |
To examine the dynamics of apical membrane redistribution
induced by ATP, resarchers used time-lapse differential interference
contrast (DIC) microscopy. Brush border cells (BBs) were immobilized within a
flow cell and imaged with the long axis of microvilli parallel to
the focal plane. Rat and mouse intestinal cells. |
| Comments |
When the
velocity of membrane movement was tallied from multiple
kymographs, researchers obtained a mean membrane translation velocity
of 19.2 ± 6.1 nm/s (Fig. 3 C). These data indicate that ATP
induces movement of apical membrane with a polarity and velocity
that are consistent with the activity of a class I myosin
(Coluccio, 1997 PMID 9277333). |
| Entered by |
Uri M |
| ID |
105556 |