Range |
~12 min at pH 9.4 - ~5 min at pH 11.2
|
Organism |
Generic |
Reference |
Shu X, Shaner NC, Yarbrough CA, Tsien RY, Remington SJ. Novel chromophores and buried charges control color in mFruits. Biochemistry. 2006 Aug 15 45(32):9639-47. p. 9641 right column bottom paragraph and p. 9642 left column top paragraphPubMed ID16893165
|
Method |
Protein samples of mStrawberry were subjected to a step
change in pH from 7.9 to 9.4 or higher, and the time dependence
of the absorbance spectra (Figure 1C) suggested a very
slow transition. Spectra at intermediate time points could be
decomposed on the basis of the endpoint spectra collected at pH 7.9 and 11.4. The fraction X(t), where X is the fraction
of the pH 7.9 baseline (blue squares in Figure 1D), could be
fit to a single exponential (blue dashed line) and a rate
constant extracted. The log of rate constants was linearly
dependent upon the final pH (red line in the inset of Figure
1D) however, a linear fit of the rate constants to the final
pH is also satisfactory. The extracted rate constants range
from 0.056 min^-1 at pH 9.4 to 0.142 min^-1 at pH 11.2. The
spectral shifts were fully reversible upon reduction of pH. Maturation times calculated according to t=ln/k |
Entered by |
Uri M |
ID |
105538 |