Number of invA mRNA copies in mid exponential phase cells
Value | 0.4 copies/CFU |
---|---|
Organism | Bacteria Salmonella enterica |
Reference | González-Escalona N, Hammack TS, Russell M, Jacobson AP, De Jesús AJ, Brown EW, Lampel KA. Detection of live Salmonella sp. cells in produce by a TaqMan-based quantitative reverse transcriptase real-time PCR targeting invA mRNA. Appl Environ Microbiol. 2009 Jun75(11):3714-20PubMed ID19376910 |
Method | DNA detection methods used for analysis of foods, are subject to the major concern of false-positive results from the detection of dead cells. To circumvent this crucial issue, a TaqMan quantitative real-time RT-PCR (qRT-PCR) assay with an RNA internal control was developed. |
Entered by | Uri M |
ID | 104212 |