Value |
106
min^-1
|
Organism |
Bacteria Staphylococcus aureus |
Reference |
Allen LM, Hodskinson MR, Sayers JR. Active site substitutions delineate distinct classes of eubacterial flap endonuclease. Biochem J. 2008 Nov 11. link PubMed ID19000038
|
Method |
Using a standard spectrophotometric assay, we quantified the exonucleolytic activity. the specific activity for T5FEN, calculated in a previous study (610 units µg-1) [29] was similar to that reported here (420±10 units µg-1), The specific activity of SaFEN (90±5 units µg-1) was approximately fivefold lower than that of T5FEN, equivalent to a turnover rate of 106 min -1. |
Comments |
the activity of E. coli FEN is approximately three-fold less. see bion 102587 |
Entered by |
Uri M |
ID |
102586 |