Range |
~2 Hours
|
Organism |
Human Homo sapiens |
Reference |
Ignowski JM, Schaffer DV. Kinetic analysis and modeling of firefly luciferase as a quantitative reporter gene in live mammalian cells. Biotechnol Bioeng. 2004 Jun 30 86(7):827-34. p.830 right column 3rd paragraphPubMed ID15162459
|
Method |
"[Researchers] have...investigated the linearity, half-life, and enzyme kinetics of
luciferase bioluminescence in live human embryonic kidney
(HEK 293) cells." |
Comments |
"The data presented in Figure 3 indicate the
enzyme decays following first-order kinetics with a half-life
of approximately 2 h. This result is faster than the value of
3 h found by Thompson et al. (1991) [BNID 105184] for the thermal decay
of luciferase in cell lysate, a discrepancy that may be due to
differences in measurements between live cells and lysates." |
Entered by |
Ron Milo - Admin |
ID |
101268 |