Concentrations of L-dopa in plasma and plasma ultrafiltrates

J Pharm Biomed Anal. 1996 Jun;14(8-10):1043-6. doi: 10.1016/s0731-7085(96)01753-0.

Abstract

A sensitive and specific procedure is described for the determination of therapeutically relevant concentrations of L-dopa in plasma and plasma ultrafiltrates (free fraction) by high performance liquid chromatography with electrochemical detection. In plasma samples from healthy adult subjects (n = 15) spiked with L-dopa (500 micrograms l-1) the free fraction averaged 76 +/- = 8% (range 61-84%). Free fraction values increased by 38% with increasing plasma concentrations of L-dopa from 100-5000 micrograms 1-1. L-dopa free fraction was not affect by the presence of 3-O-methyl-dopa at concentrations up to 10,000 micrograms l-1.

MeSH terms

  • Adult
  • Antiparkinson Agents / blood*
  • Blood Proteins / metabolism
  • Chromatography, High Pressure Liquid / methods
  • Humans
  • In Vitro Techniques
  • Levodopa / blood*
  • Protein Binding
  • Ultrafiltration

Substances

  • Antiparkinson Agents
  • Blood Proteins
  • Levodopa