NADP+-specific isocitrate dehydrogenase of Excherichia coli. III. Two-step purification employing affinity chromatography

Biochim Biophys Acta. 1976 Sep 14;445(2):280-5. doi: 10.1016/0005-2744(76)90082-6.

Abstract

The NADP+-specific isocitrate dehydrogenase (threo-DS-isocitrate:NADP+ oxidoreductase (decarboxylating), EC 1.1.1.42) of Excherichia coli has been purified to electrophoretic homogeneity by a two-step purification procedure employing affinity chromatography. The overall yield of enzyme was 30% with specific activity 125 mumol/min per ng protein. Electrophoretic homogeneity of the isocitrate dehydrogenase was deterimed in analytical polyacrylamide gels in a Tris/acetate/EDTA buffer system at pH 7.5 and in a citrate/phosphate buffer system at pH 6.0.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Chromatography, Affinity
  • Escherichia coli / enzymology*
  • Isocitrate Dehydrogenase / isolation & purification*
  • Isocitrate Dehydrogenase / metabolism
  • NADP

Substances

  • NADP
  • Isocitrate Dehydrogenase