Application of differential scanning microcalorimetry to the study of cellular processes: heat production and glucose oxidation of murine macrophages

Proc Natl Acad Sci U S A. 1981 Oct;78(10):5958-62. doi: 10.1073/pnas.78.10.5958.

Abstract

Differential scanning microcalorimetry provides a noninvasive method for studying heat evolution in living cells. We used this technique to measure the heat evolved by thioglycollate broth-elicited mouse macrophages, and the effects of NaF, KCN, cycloheximide, and cytochalasins B and D on this parameter. The total heat evolved in the interval 10--37 degrees C scanned at 1 degree C min-1 ranged from 300 to 2500 X 10(-12) cal (1 Cal = 4.184 J) per cell, depending on cell density, glucose concentration, and the presence or absence of various drugs.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Calorimetry, Differential Scanning
  • Cycloheximide / pharmacology
  • Cytochalasins / pharmacology
  • Energy Metabolism
  • Glucose / metabolism*
  • Glycolysis / drug effects*
  • Hot Temperature
  • Kinetics
  • Macrophages / drug effects
  • Macrophages / metabolism*
  • Mice
  • Potassium Cyanide / pharmacology
  • Sodium Fluoride / pharmacology

Substances

  • Cytochalasins
  • Sodium Fluoride
  • Cycloheximide
  • Glucose
  • Potassium Cyanide