Carbon monoxide-dependent chemolithotrophic growth of Clostridium thermoautotrophicum

Appl Environ Microbiol. 1987 Aug;53(8):1902-6. doi: 10.1128/aem.53.8.1902-1906.1987.

Abstract

The acetogen Clostridium thermoautotrophicum was cultivated under CO-dependent chemolithotrophic conditions. CO-dependent growth profiles and energetics indicated that supplemental CO2 was fundamental to efficient growth at the expense of CO. Overall product stoichiometry approximated 6.5CO----CH3CO2H + 3.5CO2 + 0.6 cell C + 0.5 unrecovered C. Initial CO/CO2 ratios of 2 to 4 yielded optimal doubling times and cell yields. Maximal YCO values approximated 2.5 g of cell dry weight per mol of CO consumed; YH2 was considerably lower than YCO. Cross-transfer growth experiments and protein profiles indicated differential expression of genes between CO and methanol cultures.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Carbon Dioxide / metabolism
  • Carbon Monoxide / metabolism*
  • Clostridium / growth & development*
  • Clostridium / metabolism
  • Methanol / metabolism

Substances

  • Carbon Dioxide
  • Carbon Monoxide
  • Methanol