Intracellular PPi concentration is not directly dependent on amount of inorganic pyrophosphatase in Escherichia coli K-12 cells

J Bacteriol. 1989 Aug;171(8):4498-500. doi: 10.1128/jb.171.8.4498-4500.1989.

Abstract

No correlation was observed between the level of inorganic pyrophosphatase (PPase) and the intracellular concentration of PPi in Escherichia coli cells. In exponentially growing cells the intracellular PPi concentration was in every case 1.5 nmol/mg (dry weight) or about 0.5 mM, even though the amount of PPase was varied from 15 to 2,600% of the control amount by mutation or by using a multicopy plasmid with an inserted gene (ppa) encoding PPase. The PPi concentration could, however, be increased or decreased from the control level under some stressful conditions.

MeSH terms

  • Diphosphates / metabolism*
  • Escherichia coli / enzymology
  • Escherichia coli / growth & development
  • Escherichia coli / metabolism*
  • Inorganic Pyrophosphatase
  • Kinetics
  • Pyrophosphatases / metabolism*

Substances

  • Diphosphates
  • Pyrophosphatases
  • Inorganic Pyrophosphatase