A critique of methods for temperature imaging in single cells

Nat Methods. 2014 Sep;11(9):899-901. doi: 10.1038/nmeth.3073.

Abstract

We argue that standard thermodynamic considerations and scaling laws show that a single cell cannot substantially raise its temperature by endogenous thermogenesis. This statement seriously questions the interpretations of recent work reporting temperature heterogeneities measured in single living cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms*
  • Animals
  • Artifacts*
  • Cell Physiological Phenomena*
  • Computer Simulation
  • Humans
  • Models, Biological*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Thermography / methods*