Rre37 stimulates accumulation of 2-oxoglutarate and glycogen under nitrogen starvation in Synechocystis sp. PCC 6803

Ancy Joseph; Shimpei Aikawa; Kengo Sasaki; Hiroshi Teramura; Tomohisa Hasunuma; Fumio Matsuda; Takashi Osanai; Masami Yokota Hirai; Akihiko Kondo

doi:10.1016/j.febslet.2013.12.008

Rre37 stimulates accumulation of 2-oxoglutarate and glycogen under nitrogen starvation in Synechocystis sp. PCC 6803

FEBS Lett. 2014 Jan 31;588(3):466-71. doi: 10.1016/j.febslet.2013.12.008. Epub 2013 Dec 24.

Authors

Ancy Joseph¹, Shimpei Aikawa¹, Kengo Sasaki², Hiroshi Teramura¹, Tomohisa Hasunuma³, Fumio Matsuda⁴, Takashi Osanai⁵, Masami Yokota Hirai⁶, Akihiko Kondo⁷

Affiliations

¹ Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 657-8501, Japan.
² Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 657-8501, Japan.
³ Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 657-8501, Japan; Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency, 3-5 Sanbancho, Chiyoda-ku, Tokyo 102-0075, Japan.
⁴ RIKEN Biomass Engineering Program, 1-7-22, Suehirocho, Tsurumi, Yokohama 230-0045, Japan; Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University,1-5 Yamadaoka, Suita, Osaka 565-0871, Japan.
⁵ PRESTO, Japan Science and Technology Agency (JST), 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.
⁶ RIKEN Center for Sustainable Resource Science, 1-7-22, Suehirocho, Tsurumi, Yokohama 230-0045, Japan.
⁷ Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 657-8501, Japan; RIKEN Biomass Engineering Program, 1-7-22, Suehirocho, Tsurumi, Yokohama 230-0045, Japan. Electronic address: akondo@kobe-u.ac.jp.

PMID: 24374346
DOI: 10.1016/j.febslet.2013.12.008

Abstract

Rre37 (sll1330) in a cyanobacterium Synechocystis sp. PCC 6803 acts as a regulatory protein for sugar catabolic genes during nitrogen starvation. Low glycogen accumulation in Δrre37 was due to low expression of glycogen anabolic genes. In addition to low 2-oxoglutarate accumulation, normal upregulated expression of genes encoding glutamate synthases (gltD and gltB) as well as accumulation of metabolites in glycolysis (fructose-6-phosphate, fructose-1,6-bisphosphate, and glyceraldehyde-3-phosphate) and tricarboxylic acid (TCA) cycle (oxaloacetate, fumarate, succinate, and aconitate) were abolished by rre37 knockout. Rre37 regulates 2-oxoglutarate accumulation, glycogen accumulation through expression of glycogen anabolic genes, and TCA cycle metabolites accumulation.

Keywords: 2-OG; 2-Oxoglutarate; 2-oxoglutarate; Cyanobacteria; GOGAT; GS; Glycogen; HPLC; LC/QqQ-MS; Nitrogen starvation; OD; Rre37; TCA; glutamate synthase; glutamine synthetase; high performance liquid chromatography; liquid chromatography/triple quadrupole mass spectrometry; optical density; tricarboxylic acid.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Citric Acid Cycle*
DNA-Binding Proteins
Fructosephosphates / metabolism
Gene Expression Regulation, Bacterial
Glutamate Synthase / metabolism*
Glycogen / metabolism*
Glycolysis
Ketoglutaric Acids / metabolism*
Nitrogen / metabolism
Starvation
Synechocystis / metabolism

Substances

DNA-Binding Proteins
Fructosephosphates
Ketoglutaric Acids
fructose-6-phosphate
Glycogen
Glutamate Synthase
Nitrogen