Solid immersion facilitates fluorescence microscopy with nanometer resolution and sub-ångström emitter localization

Dominik Wildanger; Brian R Patton; Heiko Schill; Luca Marseglia; J P Hadden; Sebastian Knauer; Andreas Schönle; John G Rarity; Jeremy L O'Brien; Stefan W Hell; Jason M Smith

doi:10.1002/adma.201203033

Solid immersion facilitates fluorescence microscopy with nanometer resolution and sub-ångström emitter localization

Adv Mater. 2012 Nov 20;24(44):OP309-13. doi: 10.1002/adma.201203033. Epub 2012 Sep 12.

Authors

Dominik Wildanger¹, Brian R Patton, Heiko Schill, Luca Marseglia, J P Hadden, Sebastian Knauer, Andreas Schönle, John G Rarity, Jeremy L O'Brien, Stefan W Hell, Jason M Smith

Affiliation

¹ Department of NanoBiophotonics, Max Planck Institut for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany. dominik@wildanger.net.

Abstract

Exploring the maximum spatial resolution achievable in far-field optical imaging, we show that applying solid immersion lenses (SIL) in stimulated emission depletion (STED) microscopy addresses single spins with a resolution down to 2.4 ± 0.3 nm and with a localization precision of 0.09 nm.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Equipment Design
Equipment Failure Analysis
Image Enhancement / instrumentation*
Lenses*
Microscopy, Fluorescence / instrumentation*
Nanotechnology / instrumentation*
Solutions

Substances

Solutions