Abstract
Mitochondrial structure and distribution are regulated by division and fusion events. Mitochondrial division is regulated by Dnm1/Drp1, a dynamin-related protein that forms helices around mitochondria to mediate fission. Little is known about what determines sites of mitochondrial fission within the mitochondrial network. The endoplasmic reticulum (ER) and mitochondria exhibit tightly coupled dynamics and have extensive contacts. We tested whether ER plays a role in mitochondrial division. We found that mitochondrial division occurred at positions where ER tubules contacted mitochondria and mediated constriction before Drp1 recruitment. Thus, ER tubules may play an active role in defining the position of mitochondrial division sites.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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COS Cells
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Chlorocebus aethiops
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Dynamins
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Endoplasmic Reticulum / physiology*
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Endoplasmic Reticulum / ultrastructure*
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GTP Phosphohydrolases / genetics
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GTP Phosphohydrolases / metabolism
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Humans
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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Microscopy, Electron
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Microscopy, Fluorescence
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Microtubule-Associated Proteins / genetics
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Microtubule-Associated Proteins / metabolism
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Mitochondria / physiology*
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Mitochondria / ultrastructure
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Mitochondrial Proteins / genetics
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Mitochondrial Proteins / metabolism
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Saccharomyces cerevisiae / ultrastructure*
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism
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Transfection
Substances
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Membrane Proteins
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Mff protein, human
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Microtubule-Associated Proteins
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Mitochondrial Proteins
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Saccharomyces cerevisiae Proteins
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GTP Phosphohydrolases
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DNM1 protein, S cerevisiae
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DNM1L protein, human
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Dynamins