Photosystem 2 effective fluorescence cross-section of cyanobacterium Synechocystis sp. PCC6803 and its mutants

J Photochem Photobiol B. 2011 Jul-Aug;104(1-2):285-91. doi: 10.1016/j.jphotobiol.2011.02.011. Epub 2011 Feb 21.

Abstract

The effective fluorescence cross-section of photosystem 2 (PS2) was defined by measurements of chlorophyll a fluorescence induction curves for the wild type of the unicellular cyanobacterium Synechocystis sp. PCC6803, C-phycocyanin deficient mutant (CK), and mutant that totally lacks phycobilisomes (PAL). It was shown that mutations lead to a strong decrease of the PS2 effective fluorescence cross-section. For instance, the effective fluorescence cross-section of PS2 for wild type, CK and PAL mutants excited at λ(ex)=655 nm were found to be 896, 220 and 83 Å(2) respectively. Here we present an estimation of energy transfer efficiency from phycobilisomes to the pigment-protein complexes of PS2. It was shown that the PS2 fluorescence enhancement coefficient reaches a maximum value of 10.7 due to the energy migration from phycobilisomes. The rate constant of energy migration was found to be equal to 1.04 × 10(10) s(-1).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chlorophyll / chemistry
  • Chlorophyll / metabolism
  • Chlorophyll A
  • Energy Transfer
  • Mutation
  • Photosystem II Protein Complex / chemistry
  • Photosystem II Protein Complex / metabolism*
  • Phycobilisomes / metabolism
  • Phycocyanin / genetics
  • Phycocyanin / metabolism*
  • Spectrometry, Fluorescence
  • Synechocystis / enzymology
  • Synechocystis / genetics
  • Synechocystis / metabolism*

Substances

  • Photosystem II Protein Complex
  • Phycobilisomes
  • Phycocyanin
  • Chlorophyll
  • Chlorophyll A