Assessment of carotenoid production by Dunaliella salina in different culture systems and operation regimes

J Biotechnol. 2011 Jan 20;151(2):180-5. doi: 10.1016/j.jbiotec.2010.11.011. Epub 2010 Nov 24.

Abstract

The effect of operation regime and culture system on carotenoid productivity by the halotolerant alga Dunaliella salina has been analyzed. Operation strategies tested included batch and semi continuous regime, as well as a two-stage approach run simultaneously in both, open tanks and closed reactor. The best results were obtained with the closed tubular photobioreactor. The highest carotenoid production (328.8 mg carotenoid l⁻¹ culture per month) was achieved with this culture system operated following the two-stage strategy. Also, closed tubular photobioreactor provided the highest carotenoid contents (10% of dry weight) in Dunaliella biomass and β-carotene abundance (90% of total carotenoids) as well as the highest 9-cis to all-trans β-carotene isomer ratio (1.5 at sunrise).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bioreactors
  • Biotechnology / methods
  • Carotenoids / chemistry*
  • Carotenoids / metabolism
  • Chlorophyta / metabolism*
  • Culture Media / metabolism*
  • Isomerism
  • Light
  • Lutein / chemistry
  • Photobioreactors*
  • Photochemistry / methods
  • Protein Isoforms
  • Temperature
  • beta Carotene / chemistry

Substances

  • Culture Media
  • Protein Isoforms
  • beta Carotene
  • Carotenoids
  • Lutein