Identification of phagocytic cells, NK-like cytotoxic cell activity and the production of cellular exudates in the coelomic cavity of adult zebrafish

Dev Comp Immunol. 2009 Oct;33(10):1077-87. doi: 10.1016/j.dci.2009.05.009. Epub 2009 Jun 6.

Abstract

Coelomic cavity (CC) cells of mature zebrafish harvested by lavage with media or trypsin-EDTA contained 0.80-1.20 x 10(5) and 2.0-3.5 x 10(5) cells, respectively. Media lavage was composed of granulocytes (60-80%), lymphocytes (10-20%), and NCC (4-10%). Granulocytes had large electron dense cytoplasmic paracrystalline granules and a segmented nucleus; they expressed plastin-1, myeloid specific peroxidase and MCSF mRNA; and they were NCAMP-1(+). Lymphocytes had B- and T-cell specific mRNA and were NCAMP-1(-) and NCCRP-1(-). NCC were 3 microm, NCAMP-1(+) and NCCRP-1(+) and did not express B- and T-cell specific mRNA. Additionally, trypsin lavage contained monocytes (marginated chromatin, low nuclear:cytoplasm ratio, sparse cytosolic granules) and macrophages (non-segmented nuclei, no margination of chromatin, abundant electron dense granules). E. coli injected into the CC were phagocytosed in a dose and time dependent fashion by granulocytes, monocytes and macrophages. NCC lysed mammalian target cells and NCAMP-1 expressing hybridoma cells in redirected lysis assays.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Abdominal Cavity
  • Animals
  • Cell Line, Tumor
  • Cells, Cultured
  • Cytotoxicity Tests, Immunologic
  • Cytotoxicity, Immunologic / immunology*
  • Escherichia coli / immunology
  • Exudates and Transudates / metabolism*
  • Female
  • Flow Cytometry
  • Gene Expression
  • HL-60 Cells
  • Humans
  • K562 Cells
  • Killer Cells, Natural / cytology
  • Killer Cells, Natural / immunology
  • Killer Cells, Natural / metabolism
  • Leukocytes / cytology
  • Leukocytes / immunology
  • Leukocytes / metabolism
  • Microscopy, Confocal
  • Microscopy, Electron
  • Phagocytes / cytology
  • Phagocytes / immunology*
  • Phagocytes / ultrastructure
  • Phagocytosis / immunology
  • Receptors, Antigen / genetics
  • Receptors, Antigen / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Zebrafish / immunology*
  • Zebrafish / metabolism
  • Zebrafish Proteins / genetics
  • Zebrafish Proteins / metabolism

Substances

  • Receptors, Antigen
  • Zebrafish Proteins
  • nonspecific cytotoxic cell receptor protein 1