Self-assembled monolayer for low density lipoprotein detection

J Mol Recognit. 2008 Nov-Dec;21(6):419-24. doi: 10.1002/jmr.922.

Abstract

Heparin (HEP) has been covalently immobilized onto 4-aminothiophenol (ATP) self-assembled monolayer (SAM) deposited onto gold (Au)-coated glass plate for low density lipoprotein (LDL) detection. The HEP/ATP/Au and LDL/HEP/ATP/Au electrodes have been characterized using cyclic voltammetry (CV) and scanning electron microscopy (SEM). Surface plasmon resonance (SPR) measurements reveal that HEP/ATP/Au electrode is sensitive to detection of the LDL in the range 0.03 microM (10 mg/dl)-0.39 microM (130 mg/dl). The values of association and dissociation rate constants in the association phase calculated by kinetic analysis have been found to be k(a) = 9.67 x 10(1) M(-1) s(-1) and k(d) = 2.64 x 10(-4) s(-1).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Electrochemistry
  • Electrodes
  • Gold / chemistry
  • Heparin / chemistry
  • Lipoproteins, LDL / analysis*
  • Lipoproteins, LDL / chemistry*
  • Microscopy, Electron, Scanning
  • Surface Plasmon Resonance

Substances

  • Lipoproteins, LDL
  • Gold
  • Heparin