Abstract
The rate of in vivo transcription elongation for Escherichia coli rRNA operons was determined by electron microscopy following addition of rifampin to log-phase cultures. Direct observation of RNA polymerase positions along rRNA operons 30, 40, and 70 s after inhibition of transcription initiation yielded a transcription elongation rate of 42 nucleotides per s.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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DNA-Directed RNA Polymerases / drug effects
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DNA-Directed RNA Polymerases / metabolism*
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Escherichia coli / drug effects
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Escherichia coli / genetics*
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Escherichia coli / ultrastructure
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Kinetics
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Microscopy, Electron
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RNA, Bacterial / genetics
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RNA, Ribosomal / genetics*
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Rifampin / pharmacology
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Transcription, Genetic / drug effects
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Transcription, Genetic / genetics*
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rRNA Operon / genetics
Substances
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RNA, Bacterial
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RNA, Ribosomal
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DNA-Directed RNA Polymerases
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Rifampin