Dual feedback loops in the GAL regulon suppress cellular heterogeneity in yeast

Nat Genet. 2006 Sep;38(9):1082-7. doi: 10.1038/ng1869. Epub 2006 Aug 27.

Abstract

Transcriptional noise is known to be an important cause of cellular heterogeneity and phenotypic variation. The extent to which molecular interaction networks may have evolved to either filter or exploit transcriptional noise is a much debated question. The yeast genetic network regulating galactose metabolism involves two proteins, Gal3p and Gal80p, that feed back positively and negatively, respectively, on GAL gene expression. Using kinetic modeling and experimental validation, we demonstrate that these feedback interactions together are important for (i) controlling the cell-to-cell variability of GAL gene expression and (ii) ensuring that cells rapidly switch to an induced state for galactose uptake.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Computer Simulation
  • Feedback, Physiological*
  • Galactose / genetics*
  • Galactose / metabolism
  • Gene Expression Regulation, Fungal
  • Models, Genetic
  • Regulon*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism

Substances

  • Galactose