Estimation of the biofilm formation of Escherichia coli K-12 by the cell number

Naoki Narisawa; Soichi Furukawa; Hirokazu Ogihara; Makari Yamasaki

doi:10.1263/jbb.99.78

Estimation of the biofilm formation of Escherichia coli K-12 by the cell number

J Biosci Bioeng. 2005 Jan;99(1):78-80. doi: 10.1263/jbb.99.78.

Authors

Naoki Narisawa¹, Soichi Furukawa, Hirokazu Ogihara, Makari Yamasaki

Affiliation

¹ Department of Food Science and Technology, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa-shi 252-8510, Japan.

PMID: 16233759
DOI: 10.1263/jbb.99.78

Abstract

We developed a method of estimating the biofilm formation of Escherichia coli K-12 strains in microtiter-plate wells by the cell number. Regression lines between the cell number and absorbance of crystal violet that stained the E. coli biofilm consisted of high and low slope lines, respectively.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Algorithms*
Biofilms / growth & development*
Colony Count, Microbial / methods*
Escherichia coli K12 / cytology
Escherichia coli K12 / isolation & purification*
Escherichia coli K12 / physiology*
Gentian Violet*
Spectrum Analysis / methods*

Substances

Gentian Violet