Mucispirillum schaedleri gen. nov., sp. nov., a spiral-shaped bacterium colonizing the mucus layer of the gastrointestinal tract of laboratory rodents

Bronwyn R Robertson; Jani L O'Rourke; Brett A Neilan; Peter Vandamme; Stephen L W On; James G Fox; Adrian Lee

doi:10.1099/ijs.0.63472-0

Mucispirillum schaedleri gen. nov., sp. nov., a spiral-shaped bacterium colonizing the mucus layer of the gastrointestinal tract of laboratory rodents

Int J Syst Evol Microbiol. 2005 May;55(Pt 3):1199-1204. doi: 10.1099/ijs.0.63472-0.

Authors

Bronwyn R Robertson¹, Jani L O'Rourke², Brett A Neilan², Peter Vandamme³, Stephen L W On⁴, James G Fox⁵, Adrian Lee²

Affiliations

¹ Ramaciotti Centre for Gene Function Analysis, The University of New South Wales, Sydney, NSW 2052, Australia.
² School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW 2052, Australia.
³ Laboratorium voor Microbiologie, Universiteit Gent, Ledeganckstraat 35, B-9000 Gent, Belgium.
⁴ Danish Veterinary Laboratory, Bülowsvej 27, DK-1790 Copenhagen V, Denmark.
⁵ Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

PMID: 15879255
DOI: 10.1099/ijs.0.63472-0

Abstract

The mammalian gastrointestinal tract is covered by a layer of mucus that can harbour a range of bacterial species specifically adapted to colonize this ecological niche. Examination of 110 bacterial isolates cultivated from the gastrointestinal tract of 23 mice revealed the presence of a subgroup of 30 isolates that did not correspond genetically with genera commonly associated with this site, i.e. members of the epsilon-Proteobacteria such as Helicobacter and Campylobacter species. Instead this group of isolates was found to lie within the phylum Deferribacteres, a completely distinct lineage in the domain Bacteria. There was a high level of consensus in results obtained from the phenotypic and genotypic characterization of a number of the isolates, which showed they were distinct from other members of the Deferribacteres. As such, they are proposed to constitute a new genus and species, Mucispirillum schaedleri gen. nov., sp. nov. These organisms are anaerobic, Gram-negative, spiral-shaped rods with bipolar flagella. The type strain is HRI I17(T) (= ATCC BAA-1009(T) = ACM 5223(T)).

MeSH terms

Anaerobiosis
Animals
Bacterial Proteins / analysis
Bacterial Proteins / isolation & purification
Campylobacter / classification
Campylobacter / genetics
DNA, Bacterial / chemistry
DNA, Bacterial / isolation & purification
DNA, Ribosomal / chemistry
DNA, Ribosomal / isolation & purification
Flagella / physiology
Gastrointestinal Tract / microbiology*
Genes, rRNA
Gentian Violet
Gram-Negative Anaerobic Straight, Curved, and Helical Rods / classification*
Gram-Negative Anaerobic Straight, Curved, and Helical Rods / cytology
Gram-Negative Anaerobic Straight, Curved, and Helical Rods / isolation & purification*
Gram-Negative Anaerobic Straight, Curved, and Helical Rods / physiology
Helicobacter / classification
Helicobacter / genetics
Mice
Molecular Sequence Data
Mucus / microbiology*
Phenazines
Phylogeny
Proteome / analysis
RNA, Bacterial / genetics
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA

Substances

Bacterial Proteins
DNA, Bacterial
DNA, Ribosomal
Gram's stain
Phenazines
Proteome
RNA, Bacterial
RNA, Ribosomal, 16S
Gentian Violet

Associated data

GENBANK/AF059186
GENBANK/AF059187
GENBANK/AF059188
GENBANK/AF059189
GENBANK/AF059190
GENBANK/AY387668
GENBANK/AY387669
GENBANK/AY387670
GENBANK/AY387671