ELECTRON MICROSCOPY OF A FISSION YEAST, SCHIZOSACCHAROMYCES POMBE

J Bacteriol. 1964 Nov;88(5):1459-66. doi: 10.1128/jb.88.5.1459-1466.1964.

Abstract

Maclean, Norman (University of Edinburgh, Edinburgh, Scotland). Electron microscopy of a fission yeast, Schizosaccharomyces pombe. J. Bacteriol. 88:1459-1466. 1964.-The structure of the fission yeast, Schizosaccharomyces pombe, was studied electron microscopically, with potassium permanganate and osmium tetroxide as fixatives. The cell was found to be bounded by a cell wall, 1,000 to 2,000 A thick, and a cell membrane. A layer of material was found between the cell membrane and the wall. The central nucleus, 2 to 3 mu in diameter, was bounded by a nuclear membrane, seen in some pictures to be double. Osmium tetroxide fixation revealed a granular body within the nucleus, identified as a nucleolus. Cytoplasmic structures included numerous vacuoles (probably normally containing lipid), a number of membranes and vesicles (which may represent a poorly organized mitochondrial system), and numerous granules (probably representing ribosomes).

MeSH terms

  • Cell Membrane*
  • Cell Nucleolus*
  • Cell Nucleus*
  • Cell Wall*
  • Cytoplasm*
  • Cytoplasmic Granules*
  • Electrons*
  • Histocytochemistry*
  • Lipids*
  • Microscopy*
  • Microscopy, Electron*
  • Mitochondria*
  • Research*
  • Ribosomes*
  • Schizosaccharomyces*
  • Scotland
  • Yeasts*

Substances

  • Lipids