Abstract
FtsH, a member of the AAA family of proteins, is the only membrane ATP-dependent protease universally conserved in prokaryotes, and the only essential ATP-dependent protease in Escherichia coli. We investigated the mechanism of degradation by FtsH. Other well-studied ATP-dependent proteases use ATP to unfold their substrates. In contrast, both in vitro and in vivo studies indicate that degradation by FtsH occurs efficiently only when the substrate is a protein of low intrinsic thermodynamic stability. Because FtsH lacks robust unfoldase activity, it is able to use the protein folding state of substrates as a criterion for degradation. This feature may be key to its role in the cell and account for its ubiquitous distribution among prokaryotic organisms.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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ATP-Dependent Proteases
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Adenosine Triphosphatases / metabolism
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Adenosine Triphosphate / metabolism
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Amino Acid Sequence
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Bacterial Proteins / chemistry*
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Bacterial Proteins / metabolism
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Cell Membrane / metabolism
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Dose-Response Relationship, Drug
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Escherichia coli / metabolism
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Escherichia coli Proteins
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Green Fluorescent Proteins
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Heat-Shock Proteins / metabolism
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Kinetics
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Luminescent Proteins / metabolism
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Membrane Proteins / chemistry*
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Membrane Proteins / metabolism
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Molecular Sequence Data
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Plasmids / metabolism
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Protein Binding
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Protein Folding
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Protein Structure, Tertiary
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RNA, Bacterial / metabolism
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Recombinant Fusion Proteins / metabolism
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Ribonucleases / metabolism
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Sigma Factor*
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Substrate Specificity
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Temperature
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Thermodynamics
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Time Factors
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Transcription Factors / metabolism
Substances
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Bacterial Proteins
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Escherichia coli Proteins
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Heat-Shock Proteins
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Luminescent Proteins
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Membrane Proteins
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RNA, Bacterial
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Recombinant Fusion Proteins
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Sigma Factor
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Transcription Factors
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heat-shock sigma factor 32
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tmRNA
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Green Fluorescent Proteins
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Adenosine Triphosphate
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Ribonucleases
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Bacillus amyloliquefaciens ribonuclease
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ATP-Dependent Proteases
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FtsH protein, E coli
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Adenosine Triphosphatases