Abstract
Transgenic tobacco plants expressing a cyanobacterial fructose-1,6/sedoheptulose-1,7-bisphosphatase targeted to chloroplasts show enhanced photosynthetic efficiency and growth characteristics under atmospheric conditions (360 p.p.m. CO2). Compared with wild-type tobacco, final dry matter and photosynthetic CO2 fixation of the transgenic plants were 1.5-fold and 1.24-fold higher, respectively. Transgenic tobacco also showed a 1.2-fold increase in initial activity of ribulose 1,5 bisphosphate carboxylase/oxygenase (Rubisco) compared with wild-type plants. Levels of intermediates in the Calvin cycle and the accumulation of carbohydrates were also higher than those in wild-type plants. This is the first report in which expression of a single plastid-targeted enzyme has been shown to improve carbon fixation and growth in transgenic plants.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Carbon / metabolism
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Chloroplasts / genetics
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Chloroplasts / metabolism
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Cloning, Molecular
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Cyanobacteria / enzymology*
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Cyanobacteria / genetics
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Fructose-Bisphosphatase / genetics*
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Fructose-Bisphosphatase / metabolism
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Fructose-Bisphosphate Aldolase / metabolism
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Glyceraldehyde-3-Phosphate Dehydrogenases / metabolism
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Nicotiana / genetics*
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Nicotiana / growth & development
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Nicotiana / metabolism
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Phosphorylation
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Phosphotransferases (Alcohol Group Acceptor) / metabolism
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Photosynthesis*
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Plant Leaves / metabolism
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Plants, Genetically Modified
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Plants, Toxic*
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Ribulose-Bisphosphate Carboxylase / metabolism
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Sugar Phosphates / genetics*
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Sugar Phosphates / metabolism
Substances
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Sugar Phosphates
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Carbon
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sedoheptulose 1,7-bisphosphate
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Glyceraldehyde-3-Phosphate Dehydrogenases
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Phosphotransferases (Alcohol Group Acceptor)
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phosphoribulokinase
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Fructose-Bisphosphatase
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Ribulose-Bisphosphate Carboxylase
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Fructose-Bisphosphate Aldolase