Abstract
In Corynebacterium glutamicum the promoter of the araBAD Escherichia coli gene is positively regulated by both arabinose and the araC gene product, as it is the case in the natural host. If the L-arabinose inducer and an active araC gene are present, significant amounts of araBAD promoter expression take place in the absence of the E. coli CRP protein. These results show that the C. glutamicum RNA polymerase is activated by the E. coli positive regulator of transcription AraC.
MeSH terms
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Aldose-Ketose Isomerases / genetics
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AraC Transcription Factor
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Arabinose / metabolism*
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Bacterial Proteins*
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Carbohydrate Epimerases / genetics
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Corynebacterium / genetics*
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Escherichia coli / enzymology
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Escherichia coli / genetics
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Escherichia coli Proteins
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Gene Expression Regulation, Bacterial*
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Genetic Engineering
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Operon*
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Phosphotransferases (Alcohol Group Acceptor) / genetics
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Promoter Regions, Genetic*
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Repressor Proteins / genetics
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Repressor Proteins / metabolism
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Transcription Factors*
Substances
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AraC Transcription Factor
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AraC protein, E coli
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Bacterial Proteins
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Escherichia coli Proteins
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Repressor Proteins
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Transcription Factors
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Arabinose
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Phosphotransferases (Alcohol Group Acceptor)
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ribulokinase
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Carbohydrate Epimerases
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L-ribulosephosphate 4-epimerase
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Aldose-Ketose Isomerases
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L-arabinose isomerase