| Range |
50-80 fmol/µg total protein
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Jishage M, Ishihama A. Regulation of RNA polymerase sigma subunit synthesis in Escherichia coli: intracellular levels of sigma 70 and sigma 38. J Bacteriol. 1995 Dec177(23):6832-5.PubMed ID7592475
|
| Primary Source |
Kawakami K, Saitoh T, Ishihama A. Biosynthesis of RNA polymerase in Escherichia coli. IX. Growth-dependent variations in the synthesis rate, content and distribution of RNA polymerase. Mol Gen Genet. 1979 Jul 13 174(2):107-16.PubMed ID386039
|
| Method |
For measurement of s subunit levels in cell extracts, a
quantitative Western blot system was developed by using monospecific
polyclonal antibodies against each s subunit. The immunostained blot was
analyzed with a PDI image analyzer system equipped with a white light scanner
to determine the intensities of s70 and s38 proteins. For each sample, the
measurement was repeated at least three times and the values shown are the
averages of three independent determinations. |
| Comments |
The concentration of s70 is maintained at a constant level,
ranging from 50 to 80 fmol per µg of total proteins throughout
the change from the logarithmic growth phase to the stationary
phase (See fig. 2 in reference, from primary source). This level for strain MC4100. The level for strain W3110 was also maintained at a constant level from the
exponential phase to the stationary phase, but was higher, 150-170 fmol/µg total protein (Jishage et al 1996, PMID: 8808934) fmol=10^-15moles |
| Entered by |
Uri M |
| ID |
105162 |