Chemical content and physiological parameters of M. extorquens AM1 cells growing on methanol (including some data on E. coli)

Range Table - link
Organism Methylobacterium extorquens
Reference Peyraud R, Schneider K, Kiefer P, Massou S, Vorholt JA, Portais JC. Genome-scale reconstruction and system level investigation of the metabolic network of Methylobacterium extorquens AM1. BMC Syst Biol. 2011 Nov 10 5: 189. doi: 10.1186/1752-0509-5-189 p.3 table 1PubMed ID22074569
Primary Source See refs beneath table
Method P.3 left column bottom paragraph: "The genome-scale (GS) metabolic network of M. extorquens AM1 was reconstructed according to previously established guidelines [ref 21]. The details of the process are given in material and methods and are schematically shown in Additional file 1 link . Briefly, the GS metabolic network was obtained by integrating relevant information collected from i) genome annotation [primary source 20], ii) published physiological, genetic and biochemical studies in M. extorquens AM1 and closely related organisms iii) biochemical information contained in databases [refs 22, 23, 24], vi) complementary investigations (biomass quantification), and intensive refinement (Additional file 2, 3). The chemical composition of M. extorquens AM1 cell was determined experimentally or taken from available literature (Table 1 and Additional file 4) and used to define the biosynthetic needs and corresponding pathways."
Comments P.7 right column top paragraph: "Taken together, the omics data were involved in the confirmation of the occurrence of 175 reactions, and the exclusion of 296 reactions. The final methylotrophic network contained 717 reactions and 722 metabolites, associated with 706 genes (Table 1, Additional file 7), and included approximately two thirds of the components of the GS [genome-scale] network." Please note: bottom-most data source in table , "Rokem et al. (1978)", wasn't located in the reference list.
Entered by Uri M
ID 114580