Frequency of gene transfer in the environment (open ocean)

Range 0.0067-0.47 Table - link cfu^-1
Organism Bacteria Roseovarius nubinhibens
Reference McDaniel LD, Young E, Delaney J, Ruhnau F, Ritchie KB, Paul JH. High frequency of horizontal gene transfer in the oceans. Science. 2010 Oct 1330(6000):50. right column bottom paragraph and table 1PubMed ID20929803
Method To document gene transfer frequencies, researchers subjected wild-type strains or natural communities from a range of environments to treatment with donor strain GTAs (Gene Transfer Agent) and documented the rates of GTA-mediated gene transfer of kanamycin resistance. The gene transfer frequencies were calculated by dividing the number of antibiotic resistant cells by the total number of recipient cells in the culture (S6), both measured as colony-forming units (cfu)/ml.
Comments Environmental gene transfer frequencies ranging from 6.7×10^-3 to 4.7×10^-1 (Table 1) are 1900 to 459 million times the frequency for transformation (Williams et al., 1997 PMID 9284562) and 650,000 to 31 million times the frequency of transduction previously measured in the marine environment (Jiang et al., 1998 PMID 9687430).
Entered by Uri M
ID 105848