ATP concentration

Value 1.54 mM Range: ±1.22 Table - link mM
Organism Bacteria Escherichia coli
Reference Yaginuma et al., Diversity in ATP concentrations in a single bacterial cell population revealed by quantitative single-cell imaging. Sci Rep. 2014 Oct 6 4 :6522. doi: 10.1038/srep06522. p.4 right column bottom paragraph & p.5 table 1 & right column 2nd paragraphPubMed ID25283467
Method P.2 left column 2nd paragraph: "Design of the new single FP[fluorescent protein]-based ATP indicator ‘‘QUEEN’’: Inspired by the Ca2+ biosensor PeriCam [ref 16] and G-CaMP [ref 17], circularly-permuted enhanced green fluorescent protein (cpEGFP)[ref 18] was inserted between 2 alpha-helices of the bacterial FoF1-ATP synthase epsilon subunit (Fig. 1a, b, also see Supplementary Note for details). 2- Amino acid (a.a.) linkers were inserted at each joint region. [Researchers] named this series of biosensors ‘‘QUEEN’’ (for QUantitative Evaluator of cellular ENergy)." P.2 right column top paragraph: "QUEEN-2m was designed as a low affinity ATP indicator so it is suitable for measuring physiological ATP levels (Kd~2 mM at 25°C)."
Comments P.4 right column bottom paragraph: "The statistics of the distribution were calculated by taking into account the experimental noise obtained in the previous section (Table 1). In this condition, averaged ATP concentration was calculated to be 1.54 mM. Interestingly, it was found that intracellular ATP concentrations were not uniform but significantly diverse between individual cells." P.5 right column 2nd paragraph: "Using QUEEN-2m, [researchers] successfully determined the ATP distribution in a single population of E. coli cells in a continuous culture (Fig. 4d) and calculated statistical parameters (Table 1). The estimated mean and SD values of ATP concentration in [their] condition was 1.54±1.22 mM (mean±SD), after correcting for the effect of noise in the experimental system." See note above table
Entered by Uri M
ID 111006