Jurkat cell diameter

Value 11.5 µm Range: ±1.5 Table - link µm
Organism Mammalian tissue culture cell
Reference Rosenbluth MJ, Lam WA, Fletcher DA. Force microscopy of nonadherent cells: a comparison of leukemia cell deformability. Biophys J. 2006 Apr 15 90(8):2994-3003. p.2998 table 1PubMed ID16443660
Method P.2995 right column 4th paragraph: "For cell diameter and nucleus/cytoplasm ratio measurements, a Zeiss Axiovert 200 microscope (Carl Zeiss, Thornwood, NY) with a Zeiss 100× 1.3 numerical aperture (NA) oil immersion phase objective was used. Nuclei were fluorescently stained with Hoechst 33342 (Molecular Probes, Eugene, OR) for visualization, and cell morphology was imaged with phase microscopy. Metamorph software (Molecular Devices, Downingtown, PA) was used to quantify the cross-sectional areas of the nuclei and whole cells."
Comments P.2997 right column 2nd paragraph from bottom: "[Investigators] considered three questions. First, at what deformation rates are any viscous contributions from the cytoplasm to the apparent cell elasticity minimized? Second, which model (Hertzian mechanics versus liquid droplet) more accurately describes the deformability of the cells? Third, how different are the mechanical properties of leukemia cells from myeloid and lymphoid lines when compared to each other and to normal neutrophils? Answers to the first two questions are described here in detail for the myeloid (HL60) cell line but were also obtained for the lymphoid (Jurkat) cell line and neutrophils (see Table 1 for a summary of these data, and Supplementary Material for more details on these data). The three cell types are quantitatively compared to answer the third question and provide biophysical insight into clinical complications associated with acute leukemia." Jurkat cells are human immortalized line of T lymphocyte cells
Entered by Gaurav
ID 104668