Methionine pool size

Value 0.29 µmol/g Range: ±0.07 µmol/g
Organism Bacteria Escherichia coli
Reference Yuan J, Fowler WU, Kimball E, Lu W, Rabinowitz JD. Kinetic flux profiling of nitrogen assimilation in Escherichia coli. Nat Chem Biol. 2006 Oct2(10):529-30. p. 530 table 1PubMed ID16936719
Method Researchers introduce kinetic flux profiling (KFP), a variant of metabolic flux profiling that involves monitoring the dynamics of incorporation of isotope-labeled nutrient into downstream products using LC-MS/MS. The essential concept of KFP is that intracellular metabolites become labeled when cells are switched from unlabeled to isotope labeled nutrient, and metabolites closer to the added nutrient in the metabolic network always get labeled before their downstream products.
Comments The units of g refers to gram cell dry weight. This is equivalent to approximately 50,000 molecules per cell, assuming a cell dry weight of 0.3 picograms.
Entered by Ben Marks
ID 101319