| Comments |
"Most parameters of [researchers] model have been measured (see ref. 4,
SI Text, and Tables S1 and S2 BNID 110068, 110069), but the model contains 2 unknown
parameters, the dissociation constant for nonspecific RNAP-DNA
binding, Kns, and the maturation time t of newly synthesized
RNAPs. [They] assume these parameters are independent of the
growth rate themselves and determine them by matching the
fraction of cytoplasmic RNAPs predicted by [their] model to data
from minicell experiments (12, 13) (see SI Text and Fig. S1). This
procedure leads to a maturation time t of 3.4 min and a dissociation
constant for nonspecific binding of 3,100µM (BNID 110064). These values are
consistent with experimental data..." |