| Comments |
"According to the rather limited photostability
of GFPem,[ref 16] [investigators] observe in most cases photobleaching
after a few exposures (Figure 3 a) and in some cases also
photoblinking. The time course of emerging fluorescent
GFPem molecules is satisfactorily fitted by a single exponential
(red line in Figure 3c). The corresponding characteristic
time constant for the observed process is 5.3 min, which is one
of the fastest maturation times for a GFP mutant observed to
date. Typical maturation times of other GFP mutants of the
S65T type range from 15 to 45 min, whereas wild-type GFP
shows even longer maturation times on the order of 2 h.[refs 14, 15, 20]" "As the characteristic chromophore
formation requires at least 5–10 min for de novo synthesized
GFP molecules,[ref 24] [investigators] have to assume that the characteristic
time constant obtained from [their] data (5.3 min) is related to
the chromophore formation." |