Estimated total ubiquitin level in HEK 293 cells

Range ~8×10^7 (85 µM, ~500 pmol/mg protein) copies/cell
Organism Human Homo sapiens
Reference Clague MJ, Heride C, Urbé S. The demographics of the ubiquitin system. Trends Cell Biol. 2015 Apr 20. pii: S0962-8924(15)00054-9. doi: 10.1016/j.tcb.2015.03.002. p.2 left column 2nd paragraphPubMed ID25906909
Primary Source Kaiser, S.E. et al. (2011) Protein standard absolute quantification (PSAQ) method for the measurement of cellular ubiquitin pools. Nat. Methods 8, 691–696 doi: 10.1038/nmeth.1649. & Yang, X. et al. (2013) Absolute quantification of E1, ubiquitin-like proteins and Nedd8-MLN4924 adduct by mass spectrometry. Cell. Biochem. Biophys. 67, 139–147 doi: 10.1007/s12013-013-9625-5.PubMed ID21743460, 23754621
Comments p.2 left column 2nd paragraph:"Total ubiquitin levels in HEK293 cells have been estimated to be ~8 × 10^7 copies per cell (85 µM, ~500 pmol/mg protein), but measurements across several other cell types give values of ~100 pmol/mg [Primary Sources]."
Entered by Uri M
ID 111554