| Range |
Translational 0.27±0.01: Rotational 0.78±0.03 unitless
|
| Organism |
Mammalian tissue culture cell |
| Reference |
Kao HP, Abney JR, Verkman AS. Determinants of the translational mobility of a small solute in cell cytoplasm. J Cell Biol. 1993 Jan120(1):175-84. p.182 right column top paragraphPubMed ID8416987
|
| Method |
FRAP (Fluorescence recovery after photobleaching) with small fluorescent probe |
| Comments |
In Swiss 3T3 fibroblasts (mouse). See p.182 right column 1st paragraph for explanation of difference between rotational and translational diffusion coefficients (due to collisions with occluding macromolecules). "[Researchers] found
that the fluorescent probe
BCECF was mobile in cell cytoplasm
and had short photobleaching-recovery
times that yielded a translational diffusion coefficient relative
to that in water (D/D0) of 0.27±0.01.
This value is significantly less than
unity and less than the BCECF rotational diffusion coefficient
relative to that in water (D/D0)rot
of 0.78±0.03." |
| Entered by |
Uri M |
| ID |
105817 |