Translational and rotational diffusion coefficients of fluorescent probe BCECF in cytoplasm relative to water (D/D0)

Range Translational 0.27±0.01: Rotational 0.78±0.03 unitless
Organism Mammalian tissue culture cell
Reference Kao HP, Abney JR, Verkman AS. Determinants of the translational mobility of a small solute in cell cytoplasm. J Cell Biol. 1993 Jan120(1):175-84. p.182 right column top paragraphPubMed ID8416987
Method FRAP (Fluorescence recovery after photobleaching) with small fluorescent probe
Comments In Swiss 3T3 fibroblasts (mouse). See p.182 right column 1st paragraph for explanation of difference between rotational and translational diffusion coefficients (due to collisions with occluding macromolecules). "[Researchers] found that the fluorescent probe BCECF was mobile in cell cytoplasm and had short photobleaching-recovery times that yielded a translational diffusion coefficient relative to that in water (D/D0) of 0.27±0.01. This value is significantly less than unity and less than the BCECF rotational diffusion coefficient relative to that in water (D/D0)rot of 0.78±0.03."
Entered by Uri M
ID 105817